Fig 1: Changes in the levels of mGluR1, mGluR5, GluN2B, GluA1, PSD-95, and a5-GABA in mPFC, CeA, and hippocampus, 11 days after the SMIR surgery. (A) The representative Western blots and quantification of synaptic transmission-related proteins in mPFC. one-way ANOVA, mGluR1 (F = 16.24, P = 0.004), mGluR5 (F = 19.42, P = 0.0005), GluN2B (F = 16.28, P = 0.0004), GluA1 (F = 7.54, P = 0.02), PSD-95 (F = 13.36, P = 0.002), and a5-GABA (F = 8.31, P = 0.009). (B) Changes in the levels of synaptic transmission-related proteins in CeA. one-way ANOVA, mGluR1 (F = 8.23, P = 0.009), mGluR5 (F = 9.72, P = 0.003), GluN2B (F = 13.58, P = 0.0008), GluA1 (F = 9.05, P = 0.004), PSD-95 (F = 16.19, P = 0.001), and a5-GABA (F = 5.74, P = 0.01). (C) Changes in the expression levels of synaptic transmission-related proteins in the hippocampus. one-way ANOVA, mGluR1 (F = 9.03, P = 0.002), mGluR5 (F = 6.85, P = 0.01), GluN2B (F = 44.96, P < 0.0001), GluA1 (F = 15.50, P = 0.001), PSD-95 (F = 7.17, P = 0.006), and a5-GABA (F = 8.26, P = 0.009). Results are expressed as mean ± SEM; Tukey’s post-hoc tests; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; n = 3–6/group.